ELISA試劑盒發(fā)表的文獻(xiàn)集錦
更新時(shí)間:2022-11-10 點(diǎn)擊次數(shù):449次ELISA試劑盒發(fā)表的文獻(xiàn)集錦
信帆生物供應(yīng)各類elisa試劑盒,提供免費(fèi)代測,上門取樣,送貨上門服務(wù)!本公司產(chǎn)品質(zhì)量穩(wěn)定,庫存充足,現(xiàn)貨供應(yīng)。
操作步驟
從室溫平衡60min后的鋁箔袋中取出所需板條,剩余板條用自封袋密封放回4℃。
設(shè)置標(biāo)準(zhǔn)品孔和樣本孔,標(biāo)準(zhǔn)品孔各加不同濃度的標(biāo)準(zhǔn)品50μL;
待測樣本孔先加待測樣本10μL,再加樣本稀釋液40μL;
隨后標(biāo)準(zhǔn)品孔和樣本孔中每孔加入辣根過氧化物酶(HRP)標(biāo)記的檢測抗體100μL,用封板膜封住反應(yīng)孔,37℃水浴鍋恒溫箱溫育60min。
棄去液體,吸水紙上拍干,每孔加滿洗滌液,靜置1min,甩去洗滌液,吸水紙上拍干,如此重復(fù)洗板5次(也可用洗板機(jī)洗板)。
每孔加入底物A、B各50μL,37℃避光孵育15min。
每孔加入終止液50μL,15min內(nèi),在450nm波長處測定各孔的OD值。
操作注意事項(xiàng)
試劑盒保存在2-8℃,使用前室溫平衡60分鐘。從冰箱取出的濃縮洗滌液會(huì)有結(jié)晶,這屬于正?,F(xiàn)象,水浴加熱使結(jié)晶溶解后再使用。樣本在使用前也要在室溫平衡60分鐘。
實(shí)驗(yàn)中不用的板條應(yīng)立即放回自封袋中,密封(低溫干燥)保存。
預(yù)處理后的樣本無需稀釋,直接取10μL加樣即可。
嚴(yán)格按照說明書中標(biāo)明的時(shí)間、加液量及順序進(jìn)行溫育操作。
所有液體組分使用前充分搖勻。
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ELISA試劑盒發(fā)表的文獻(xiàn)集錦ELISA試劑盒發(fā)表的文獻(xiàn)集錦